Jagged1 regulates endometrial receptivity in both humans and mice

The human endometrium undergoes cycle-dependent changes and is only receptive to an implanting blastocyst within a narrow window of 2-4 days in the mid-secretory phase. Such functional require delicate interplay between diversity factors including cytokines signaling pathways. Notch pathway members are expressed endometrium. We have previously demonstrated that ligand Jagged1 (JAG1) localizes endometrial luminal epithelium (LE) abnormally reduced infertile women during receptivity. However, consequences JAG1 production on receptivity implantation unknown. This study aimed determine role regulating humans mice. Knockdown both primary epithelial cells Ishikawa significantly their adhesive capacity HTR8/SVneo (trophoblast cell line) spheroids. confirmed cells, interacted with Receptor 3 (NOTCH3) knockdown expression downstream target HEY1 classical markers. Jag1 mouse LE impaired implantation. identified ten genes (related tight junction, infertility, adhesion) were differentially by Further analysis junction family species revealed altered components Together, our data regulated mice likely via different mechanisms.